Published on 17 July 2012
Research Areas
Agrobiodiversity, CropsCountries
Colombia, UgandaTimeline
Start Date: 1 July 2011 | End Date: 30 June 2012Overview
In this project, the objective is to produce doubled haploids in cassava and banana/plantain using centromere engineering. Produce Agrobacterium binary vectors to replace endogenous CENH3 with altered variants in cassava and banana. Create transgenic cassava and banana lines expressing altered CENH3 proteins in place of endogenous CENH3 (putative haploid inducers). Cross putative haploid inducing lines to wild-type cassava and banana, and assay for haploid progeny.
Was the project successful in obtaining a viable haploid inducer