BREAD: Fast breeding for slow crops: doubled haploids in cassava and banana

Published on 21 January 2014

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Timeline

Start Date: 1 July 2012 | End Date: 30 June 2013

Overview

This project aims to:

  • Produce doubled haploids in cassava and banana/plantain using centromere engineering
  • Produce Agrobacterium binary vectors to replace endogenous CENH3 with altered variants in cassava and banana
  • Create transgenic cassava and banana lines expressing altered CENH3 proteins in place of endogenous CENH3 (putative haploid inducers).
  • Cross putative haploid inducing lines to wild-type cassava and banana, and assay for haploid progeny

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